Название: The hunt for THE hidden biodiversity IN SULFIDIC CAVES: A case study oF unique Chloroflexi affiliated species and other novel species from Lower Kane Cave, WYoming, USA
Источник: 15th International Congress of Speleology
Место публикации: Germany
Том:
Выпуск:
Страницы: 395-396
Web/Url:
№ полки:
Добавлено: Elmaz
Ключевые слова: biodiversity, sulfidic caves, США, микробиология, экосистема пещер
Источник:
Реферат: Lower Kane Cave (LKC), Wyoming, USA, is a sulfidic cave system containing microbial mats that are structured with filamentous and web-like morphotypes at the mat surface. Our main research objective in LKC is to investigate its microbial diversity and ecosystem function, and to explore basic principles of biologic and geologic controls
of microbial diversity and ecosystem function in a sulfurbased terrestrial subsurface. Our previous work focused on characterizing the diversity of the microbial communities,
which are dominated by putative chemolithoautotrophic, sulfur-oxidizing groups within the Epsilonproteobacteria and Gammaproteobacteria from the aerobic portions of the mats.
In contrast, the anaerobic mat interior has an unexpectedly diverse microbial community dominated by unculturable members of the Deltaproteobacteria, Chloroflexi-affiliated
bacteria, and novel candidate groups. The function of most of these putative anaerobic groups is not yet known. An intense approach, consisting of a comparative phylogeneticbiogeographic inventory of all relevant relatives retrieved from subsurface systems, in particular caves and anaerobic, oligotrophic environments, 16S rRNA-based, DGGE screening, 16S rRNA-based clone libraries, standard fluorescence in situ hybridization, CARD-FISH, and different enrichment studies using microbial mat samples
from throughout the cave, was undertaken to trace some of the novel groups, in particular the novel Chloroflexiaffiliated species, in more detail