• Библ. индекс 18673
  • Тип материала: S
  • Автор(ы): SAHL J.W., HARRIS J.K.
  • Редактор(ы): 
  • Год: 2009
  • Название: AN AUTONOMOUS ROBOTIC EXPLORATION OF DEEP PHREATIC SINKHOLES REVEALS A WEALTH OF MICROBIAL DIVERSITY
  • Источник: 15th International Congress of Speleology
  • Место публикации: Colorado
  • Том: 
  • Выпуск: 
  • Страницы: 408-409
  • Web/Url: 
  • № полки: 
  • Добавлено: Elmaz
  • Ключевые слова: ROBOTIC EXPLORATION, PHREATIC SINKHOLES, MICROBIAL DIVERSITY, микробиология, Мексика
  • Источник: 
  • Реферат:
    In spring of 2007, the deep phreatic thermal explorer (DEPTHX), an autonomous underwater vehicle, explored several phreatic limestone sinkholes (cenotes) in Northeastern Mexico as part of a NASA funded project to one day search for extraterrestrial microbial life.During dive missions in the cenote, the vehicle collected water column and biomat samples at a range of depths and aspects.Samples were returned to the surface, collected, DNA was extracted from each sample, and a molecular survey of the microbial community present was conducted using 16S rRNA gene analysis. Further analysis on extracted DNA samples was carried out with a barcoded amplicon pyrosequencing approach (Hamady et al., 2008) to reveal higher resolution microbial community structure information.