Название: AN AUTONOMOUS ROBOTIC EXPLORATION OF DEEP PHREATIC SINKHOLES REVEALS A WEALTH OF MICROBIAL DIVERSITY
Источник: 15th International Congress of Speleology
Место публикации: Colorado
Том:
Выпуск:
Страницы: 408-409
Web/Url:
№ полки:
Добавлено: Elmaz
Ключевые слова: ROBOTIC EXPLORATION, PHREATIC SINKHOLES, MICROBIAL DIVERSITY, микробиология, Мексика
Источник:
Реферат: In spring of 2007, the deep phreatic thermal explorer (DEPTHX), an autonomous underwater vehicle, explored several phreatic limestone sinkholes (cenotes) in Northeastern Mexico as part of a NASA funded project to one day search for extraterrestrial microbial life.During dive missions in the cenote, the vehicle collected water column and biomat samples at a range of depths and aspects.Samples were returned to the surface, collected, DNA was extracted from each sample, and a molecular survey of the microbial
community present was conducted using 16S rRNA gene analysis. Further analysis on extracted DNA samples was carried out with a barcoded amplicon pyrosequencing approach (Hamady et al., 2008) to reveal higher resolution microbial community structure information.